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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMB Monoclonal antibody, PBS Only

GSDMB Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67527-1-PBS

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of MCF-7 using 67527-1-Ig (same clone as 67527-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of PC-3 using 67527-1-Ig (same clone as 67527-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of COLO 320 using 67527-1-Ig (same clone as 67527-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    IF Staining of HepG2 using 67527-1-Ig (same clone as 67527-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSDMB antibody (67527-1-Ig, Clone: 2F12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67527-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67527-1-PBS targets GSDMB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSDMB fusion protein Ag28332 相同性解析による交差性が予測される生物種
Full Name gasdermin B
Calculated molecular weight 411 aa, 47 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC025682
Gene symbol GSDMB
Gene ID (NCBI) 55876
RRIDAB_2882746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.