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Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using CoraLite® Plus 488-conjugated GSDMD Recombinant an (CL488-80918)

    FC experiment of RAW 264.7 using CL488-80918

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GSDMD (CL488-80918, Clone:2N24) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated GSDMD Recombinant antibody

GSDMD Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-80918
Clone No.2N24

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Synonyms

DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80918 targets GSDMD in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMD fusion protein Ag14757 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight 484 aa, 53 kDa
Observed molecular weight50-53 kDa
GenBank accession numberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_3084492
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GSDMD antibody CL488-80918Download protocol
Standard Protocols
Click here to view our Standard Protocols