CoraLite® Plus 647-conjugated GSK3B Monoclonal antibody

GSK3B Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2E9E4

Cat no : CL647-67329

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1


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Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67329 targets GSK3B in FC (Intra) applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSK3B fusion protein Ag17320 相同性解析による交差性が予測される生物種
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 46-48 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GSK3B antibody CL647-67329Download protocol
Standard Protocols
Click here to view our Standard Protocols