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GSNOR,ADH5 Monoclonal antibody

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66193-1-Ig
Clone No.5E6E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, CoIP, ELISA

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHuH-7 cells, human testis tissue, HepG2 cells, fetal human brain tissue, pig liver tissue, rat brain tissue, rat liver tissue
Positive IHC detected inhuman pancreas cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66193-1-Ig targets GSNOR,ADH5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag9511 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 374 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2881587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Protocols

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 66193-1-IgDownload protocol
IHC protocol for GSNOR,ADH5 antibody 66193-1-IgDownload protocol
IF protocol for GSNOR,ADH5 antibody 66193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Psychiatry

Degradation of FA reduces Aβ neurotoxicity and Alzheimer-related phenotypes.

Authors - Xuechao Fei
mouseIF,WB

Arterioscler Thromb Vasc Biol

Arginase-1 Deletion in Erythrocytes Promotes Vascular Calcification via Enhanced GSNOR (S-Nitrosoglutathione Reductase) Expression and NO Signaling in Smooth Muscle Cells

Authors - Rajinikanth Gogiraju
ratIF

Front Physiol

Maintenance of Intestinal Homeostasis in Diarrhea-Predominant Irritable Bowel Syndrome by Electroacupuncture Through Submucosal Enteric Glial Cell-Derived S-Nitrosoglutathione

Authors - Yujun Hou
mouseIF

Alzheimers Dement (N Y)

New insight into Alzheimer's disease: Light reverses Aβ-obstructed interstitial fluid flow and ameliorates memory decline in APP/PS1 mice.

Authors - Xiangpei Yue
mouseIF,CoIP

Eur J Pharmacol

N6022 attenuates cerebral ischemia/reperfusion injury-induced microglia ferroptosis by promoting Nrf2 nuclear translocation and inhibiting the GSNOR/GSTP1 axis

Authors - Wan-Li Duan
mouseWB

FASEB J

Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment

Authors - Kenneth N Maclean
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