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Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of fetal human brain using 66193-1-Ig (same clone as 66193-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of rat brain using 66193-1-Ig (same clone as 66193-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of rat liver using 66193-1-Ig (same clone as 66193-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of pig liver using 66193-1-Ig (same clone as 66193-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Monoclonal antibody, PBS Only

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66193-1-PBS
Clone No.5E6E2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66193-1-PBS targets GSNOR,ADH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag9511 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 374 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2881587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11766
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).