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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Monoclonal antibody, PBS Only

GSNOR,ADH5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E6E2

Cat no : 66193-1-PBS

Synonyms

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

Validation Data Gallery

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  • Western Blot (WB) analysis of HuH-7 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HuH-7 using 66193-1-Ig (same clone as 66193-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    WB result of GSNOR,ADH5 antibody (66193-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSNOR,ADH5 transfected HepG2 cells. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of rat brain using 66193-1-Ig (same clone as 66193-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of rat liver using 66193-1-Ig (same clone as 66193-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of pig liver using 66193-1-Ig (same clone as 66193-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    WB analysis of fetal human brain using 66193-1-Ig (same clone as 66193-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human pancreas cancer using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IHC staining of human testis using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66193-1-Ig (GSNOR,ADH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSNOR,ADH5 Monoclonal antibody (66193-1-Ig)

    IF Staining of HepG2 using 66193-1-Ig (same clone as 66193-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66193-1-Ig (GSNOR,ADH5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66193-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66193-1-PBS targets GSNOR,ADH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSNOR,ADH5 fusion protein Ag9511 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Calculated molecular weight 374 aa, 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014665
Gene symbol ADH5
Gene ID (NCBI) 128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).