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GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for WB, IP, ELISA
Cat No. 66001-2-Ig
Clone No.3G12B10

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum and More (1)

Applications

WB, IF, IP, CoIP, ChIP, ELISA

GST, GST Tag

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
HRP
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inE. coli lysate

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66001-2-Ig targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Reactivity Recombinant protein, Schistosoma japonicum
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2881488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.

Protocols

Product Specific Protocols
WB protocol for GST Tag antibody 66001-2-IgDownload protocol
IP protocol for GST Tag antibody 66001-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IP

Nat Food

Identification and knockout of a herbivore susceptibility gene enhances planthopper resistance and increases rice yield

Authors - Peng Kuai
WB

Nat Cancer

METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma

Authors - Xiangyu Zeng
WB

Nat Cell Biol

Phagolysosome resolution requires contacts with the endoplasmic reticulum and phosphatidylinositol-4-phosphate signalling.

Authors - Roni Levin-Konigsberg
WB

Sci Bull (Beijing)

Targeted clearance of mitochondria by an autophagy-tethering compound (ATTEC) and its potential therapeutic effects

Authors - Shuixia Tan
WB

Nat Commun

Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex

Authors - Zili Song
WB

Nat Commun

N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization

Authors - Weiji Weng
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