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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GST Tag Monoclonal antibody, PBS Only

GST Tag Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat no : 66001-2-PBS

Synonyms

GST, GST Tag

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using GST Tag Monoclonal antibody (66001-2-Ig)

    WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using GST Tag Monoclonal antibody (66001-2-Ig)

    WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Recombinant protein using GST Tag Monoclonal antibody (66001-2-Ig)

    IP experiment of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

    IP result of anti-GST Tag (IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66001-2-PBS targets GST Tag in WB, IP, Indirect ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

Tested Reactivity Recombinant protein, Schistosoma japonicum
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.