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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GST Tag Recombinant antibody (80006-1-RR)

    WB analysis using 80006-1-RR (same clone as 80006-1-PBS)

    GST tagged protein, target band 42 kDa and degraded band 32 kDa, and other negative samples were subjected to SDS PAGE followed by western blot with 80006-1-RR (GST Tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80006-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Recombinant protein using GST Tag Recombinant antibody (80006-1-RR)

    IP experiment of Recombinant protein using 80006-1-RR (same clone as 80006-1-PBS)

    IP result of anti-GST Tag (IP:80006-1-RR, 4ug; Detection:80006-1-RR 1:5000) with Recombinant protein lysate 1200 ug. This data was developed using the same antibody clone with 80006-1-PBS in a different storage buffer formulation.

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GST Tag Recombinant antibody, PBS Only

GST Tag Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 80006-1-PBS
Clone No.9B24

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, Indirect ELISA

Synonyms

GST, Glutathione S-Transferase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80006-1-PBS targets GST Tag in WB, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GST Tag fusion protein Ag0040 相同性解析による交差性が予測される生物種
Full Name GST Tag
Calculated molecular weight 26 kDa
GenBank accession numberFN315687
Gene Symbol
Gene ID (NCBI)
RRIDAB_2882936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.