Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
80006-1-RR targets GST Tag in WB, IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GST Tag fusion protein Ag0040 相同性解析による交差性が予測される生物種 |
| Full Name | GST Tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | FN315687 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2882936 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody is a Rabbit recombinant antibody to GST tag.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GST Tag antibody 80006-1-RR | Download protocol |
| IP protocol for GST Tag antibody 80006-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells | ||
Vet Microbiol PEDV N protein capture protein translation element PABPC1 and eIF4F to promote viral replication |