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  • Featured Product
  • KD/KO Validated

GSTK1 Recombinant antibody, PBS Only

GSTK1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3J1

Cat no : 81527-1-PBS

Synonyms

DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTK1 Recombinant antibody (81527-1-RR)

    WB analysis using 81527-1-RR (same clone as 81527-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GSTK1 Recombinant antibody (81527-1-RR)

    WB analysis of HepG2 using 81527-1-RR (same clone as 81527-1-PBS)

    WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells. This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of mouse testis using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human liver cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human pancreas cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human lung cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human stomach cancer using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of mouse liver using 81527-1-RR (same clone as 81527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSTK1 Recombinant antibody (81527-1-RR)

    IF Staining of HeLa using 81527-1-RR (same clone as 81527-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81527-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81527-1-RR

    Affinity and Kinetic Characterization of 81527-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81527-1-RR against Human GSTK1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81527-1-PBS targets GSTK1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSTK1 fusion protein Ag6036 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase kappa 1
Calculated molecular weight 32 kDa
Observed molecular weight27 kDa
GenBank accession numberBC050715
Gene symbol GSTK1
Gene ID (NCBI) 373156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).