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  • Featured Product
  • KD/KO Validated

GSTK1 Recombinant antibody

GSTK1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3J1

Cat no : 81527-1-RR

Synonyms

DsbA-L, GST 13 13, GST, Glutathione S-transferase subunit 13, Glutathione S-transferase kappa 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTK1 Recombinant antibody (81527-1-RR)

    WB analysis using 81527-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81527-1-RR (GSTK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GSTK1 Recombinant antibody (81527-1-RR)

    WB analysis of HepG2 using 81527-1-RR

    WB result of GSTK1 antibody (81527-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTK1 transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of mouse testis using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human liver cancer using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human pancreas cancer using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human lung cancer using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of human stomach cancer using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTK1 Recombinant antibody (81527-1-RR)

    IHC staining of mouse liver using 81527-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81527-1-RR (GSTK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GSTK1 Recombinant antibody (81527-1-RR)

    IF Staining of HeLa using 81527-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSTK1 antibody (81527-1-RR, Clone: 3J1 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81527-1-RR

    Affinity and Kinetic Characterization of 81527-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81527-1-RR against Human GSTK1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, L02 cells, Raji cells, Daudi cells
Positive IHC detected inmouse testis tissue, mouse liver tissue, human pancreas cancer tissue, human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81527-1-RR targets GSTK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSTK1 fusion protein Ag6036 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase kappa 1
Calculated molecular weight 32 kDa
Observed molecular weight27 kDa
GenBank accession numberBC050715
Gene symbol GSTK1
Gene ID (NCBI) 373156
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSTK1, glutathione S-transferase kappa 1, belongs to the superfamily of glutathione S-transferase (GST), which is related to oxidative stress (PMID:16081649). GSTK1 expression is negatively correlated with obesity and hypertrophic cardiomyopathy (PMID:21428694, PMID:27378925). GSTK1 was expressed in the peroxisomal and mitochondrial fractions and localized to peroxisomes (PubMed:14742434).

Protocols

Product Specific Protocols
WB protocol for GSTK1 antibody 81527-1-RRDownload protocol
IHC protocol for GSTK1 antibody 81527-1-RRDownload protocol
IF protocol for GSTK1 antibody 81527-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols