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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis using 68210-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis using 68210-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis of pig liver using 68210-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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GSTM5 Monoclonal antibody

GSTM5 Monoclonal Antibody for WB, ELISA
Cat No. 68210-1-Ig
Clone No.2E5C2
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit and More (1)

Applications

WB, ELISA

Synonyms

glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrabbit brain tissue, pig liver tissue, rabbit liver tissue, rat brain tissue, mouse brain tissue, mouse liver tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68210-1-Ig targets GSTM5 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GSTM5 fusion protein Ag6054 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 5
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC058881
Gene Symbol GSTM5
Gene ID (NCBI) 2949
RRIDAB_2935298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSTM5, Glutathione S-transferases, belongs to eukaryotic and prokaryotic phase II metabolic isozymes family. It can catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for detoxification. GSTM5 undergo epigenetic repression in AMD (age-related macular degeneration) RPE/choroid, which may increase susceptibility to oxidative stress in AMD retinas (PMID: 22410570). GSTM5 was found in brain, lung and testis (PMID: 9230131).

Protocols

Product Specific Protocols
WB protocol for GSTM5 antibody 68210-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Lasers Med Sci

Revealing the mechanism of 755-nm long-pulsed alexandrite laser in inhibiting infantile hemangioma endothelial cells through transcriptome sequencing

Authors - Chen Ke
View Article