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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis using 68210-1-Ig (same clone as 68210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis using 68210-1-Ig (same clone as 68210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68210-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GSTM5 Monoclonal antibody (68210-1-Ig)

    WB analysis of pig liver using 68210-1-Ig (same clone as 68210-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68210-1-Ig (GSTM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68210-1-PBS in a different storage buffer formulation.

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GSTM5 Monoclonal antibody, PBS Only

GSTM5 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68210-1-PBS
Clone No.2E5C2

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, Indirect ELISA

Synonyms

glutathione S transferase mu 5, GST class mu 5, GSTM5, GSTM5 5, GTM5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68210-1-PBS targets GSTM5 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GSTM5 fusion protein Ag6054 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase mu 5
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC058881
Gene Symbol GSTM5
Gene ID (NCBI) 2949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46439
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GSTM5, Glutathione S-transferases, belongs to eukaryotic and prokaryotic phase II metabolic isozymes family. It can catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for detoxification. GSTM5 undergo epigenetic repression in AMD (age-related macular degeneration) RPE/choroid, which may increase susceptibility to oxidative stress in AMD retinas (PMID: 22410570). GSTM5 was found in brain, lung and testis (PMID: 9230131).