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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GSTO1 Polyclonal antibody

GSTO1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15124

Synonyms

Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, glutathione S transferase omega 1, EC:2.5.1.18, EC:1.8.5.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using CoraLite® Plus 488-conjugated GSTO1 Polyclonal ant (CL488-15124)

    IF Staining of PC-3 using CL488-15124

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 GSTO1 antibody (CL488-15124) at dilution of 1:200.

  • Flow cytometry (FC) experiment of PC-3 cells using CoraLite® Plus 488-conjugated GSTO1 Polyclonal ant (CL488-15124)

    FC experiment of PC-3 using CL488-15124

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Gsto1 Polyclonal Antibody (CL488-15124)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15124 targets GSTO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTO1 fusion protein Ag7199 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase omega 1
Calculated molecular weight 28 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC000127
Gene symbol GSTO1
Gene ID (NCBI) 9446
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GSTO1 antibody CL488-15124Download protocol
FC protocol for CL Plus 488 GSTO1 antibody CL488-15124Download protocol
Standard Protocols
Click here to view our Standard Protocols