• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15902-1-AP

Synonyms

GST3/GST pi, GST3, Glutathione S-transferase P, FAEES3, EC:2.5.1.18

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GSTP1 Polyclonal antibody (15902-1-AP)

    WB analysis using 15902-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSTP1 Polyclonal antibody (15902-1-AP)

    WB analysis of HeLa using 15902-1-AP

    WB result of GSTP1 antibody (15902-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    WB analysis of human brain using 15902-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IP experiment of mouse brain using 15902-1-AP

    IP result of anti-GSTP1 (IP:15902-1-AP, 3ug; Detection:15902-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of human skin using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of mouse skin using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of mouse skin using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of human colon using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of human liver using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GSTP1 Polyclonal antibody (15902-1-AP)

    IHC staining of human liver using 15902-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSTP1 Polyclonal antibody (15902-1-AP)

    IF Staining of HepG2 using 15902-1-AP

    Immunofluorescent analysis of HepG2 cells using 15902-1-AP (GSTP1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using GSTP1 Polyclonal antibody (15902-1-AP)

    FC experiment of HepG2 using 15902-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GSTP1 Polyclonal antibody (15902-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inCOLO 320 cells, human brain tissue, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, PC-3 cells, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skin tissue, human colon, human liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15902-1-AP targets GSTP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTP1 fusion protein Ag8731 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase pi 1
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC010915
Gene symbol GSTP1
Gene ID (NCBI) 2950
RRIDAB_2116216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Protocols

Product Specific Protocols
WB protocol for GSTP1 antibody 15902-1-APDownload protocol
IHC protocol for GSTP1 antibody 15902-1-APDownload protocol
IF protocol for GSTP1 antibody 15902-1-APDownload protocol
IP protocol for GSTP1 antibody 15902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation

Authors - Wenxin Zhang
  • KD Validated
View Article
mouseWB

Redox Biol

GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux

Authors - Xiaoxiao Ji
  • KD Validated
View Article
mouseIF

J Cell Biol

TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.

Authors - Wan Yun Ho
View Article
mouseWB

Part Fibre Toxicol

Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.

Authors - Ming Xu
View Article
mouseWB

Glia

Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome

Authors - Sophie B Siems
View Article
mouseWB

Cell Death Dis

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Authors - Cheng Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

James (Verified Customer) (11-30-2022)

Works pretty well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Liver
GSTP1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:15902-1-AP)