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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GSTP1 Polyclonal antibody

GSTP1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-15902

Synonyms

GST3/GST pi, GST3, Glutathione S-transferase P, FAEES3, EC:2.5.1.18

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated GSTP1 Polyclonal ant (CL647-15902)

    IF Staining of HepG2 using CL647-15902

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 GSTP1 antibody (CL647-15902) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated GSTP1 Polyclonal ant (CL647-15902)

    FC experiment of HepG2 using CL647-15902

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GSTP1 (CL647-15902) (red), or 0.2 ug Rabbit IgG CL647 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-15902 targets GSTP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTP1 fusion protein Ag8731 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase pi 1
Calculated molecular weight 210 aa, 23 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC010915
Gene symbol GSTP1
Gene ID (NCBI) 2950
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 GSTP1 antibody CL647-15902Download protocol
Standard Protocols
Click here to view our Standard Protocols