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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSTT1 Monoclonal antibody

GSTT1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A4C11

Cat no : 67645-1-Ig

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis using 67645-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis of pig liver using 67645-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GSTT1 Monoclonal antibody (67645-1-Ig)

    WB analysis of K-562 using 67645-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IHC staining of mouse liver using 67645-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IHC staining of mouse liver using 67645-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IF Staining of mouse liver using 67645-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using GSTT1 Monoclonal antibody (67645-1-Ig)

    IF Staining of mouse liver using 67645-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inpig lung tissue, pig liver tissue, K-562 cells, rat liver tissue, mouse brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67645-1-Ig targets GSTT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GSTT1 fusion protein Ag8573 相同性解析による交差性が予測される生物種
Full Name glutathione S-transferase theta 1
Calculated molecular weight 240 aa, 27 kDa
Observed molecular weight 28 kDa, 30 kDa
GenBank accession numberBC007065
Gene symbol GSTT1
Gene ID (NCBI) 2952
RRIDAB_2882845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

Protocols

Product Specific Protocols
WB protocol for GSTT1 antibody 67645-1-IgDownload protocol
IHC protocol for GSTT1 antibody 67645-1-IgDownload protocol
IF protocol for GSTT1 antibody 67645-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols