Validation Data Gallery
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Tested Applications
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-67645 targets GSTT1 in IF-P applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GSTT1 fusion protein Ag8573 相同性解析による交差性が予測される生物種 |
| Full Name | glutathione S-transferase theta 1 |
| Calculated molecular weight | 240 aa, 27 kDa |
| Observed molecular weight | 28 kDa, 30 kDa |
| GenBank accession number | BC007065 |
| Gene Symbol | GSTT1 |
| Gene ID (NCBI) | 2952 |
| RRID | AB_2934774 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30711 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GSTT1 antibody CL594-67645 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |