Galectin-1 Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

WB result of Galectin-1 antibody (60223-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

human placenta tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 60223-1-Ig (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

human heart tissue were subjected to SDS PAGE followed by western blot with 60223-1-Ig (GAL1,LGALS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60223-1-Ig (Galectin-1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using Galectin-1 antibody (60223-1-Ig, Clone: 3G10D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60223-1-PBS in a different storage buffer formulation.