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Galectin-1 Monoclonal antibody
Galectin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3G10D2
Cat no : 60223-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, human placenta tissue, human heart tissue, HepG2 cells, U2OS cells, A549 cells, HeLa cells, L02 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | U2OS cells, Saos-2 cells, human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 2 publications below |
Product Information
60223-1-Ig targets Galectin-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Galectin-1 fusion protein Ag2432 相同性解析による交差性が予測される生物種 |
| Full Name | lectin, galactoside-binding, soluble, 1 |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 13 kDa |
| GenBank accession number | BC020675 |
| Gene symbol | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_11182608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| IHC protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| IF protocol for Galectin-1 antibody 60223-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Oncotarget Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin. | ||
Sci Rep Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers. | ||
Int J Oncol Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer | ||
mBio Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (05-23-2022) | It is excellent with ARPE-19 cells but gives no signal with 293T
 |
FH Sushmita (Verified Customer) (12-16-2019) | This Galactin-1 antibody is working very fine which I applied to probe in western blot in cancer cell line.
 |
