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CoraLite®594-conjugated Galectin-1 Monoclonal antibody
Galectin-1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF/ICC, IF-P, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3G10D2
Cat no : CL594-60223
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human colon cancer tissue, Saos-2 cells, U2OS cells |
Positive IF/ICC detected in | Saos-2 cells, U2OS cells |
Positive FC (Intra) detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:600-1:2400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-60223 targets Galectin-1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Galectin-1 fusion protein Ag2432 相同性解析による交差性が予測される生物種 |
Full Name | lectin, galactoside-binding, soluble, 1 |
Calculated molecular weight | 15 kDa |
Observed molecular weight | 13 kDa |
GenBank accession number | BC020675 |
Gene symbol | Galectin-1 |
Gene ID (NCBI) | 3956 |
RRID | AB_2919903 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Galectin-1 antibody CL594-60223 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |