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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated Galectin-3 Monoclonal antibody

Galectin-3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C1B2

Cat no : CL647-60207

Synonyms

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated Galectin-3 Monoclona (CL647-60207)

    IF Staining of HeLa using CL647-60207

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 Galectin-3 antibody (CL647-60207, Clone: 1C1B2 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Galectin-3 Monoclona (CL647-60207)

    FC experiment of HeLa using CL647-60207

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human Galectin-3 (CL647-60207, Clone:1C1B2) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG2b Isotype Control (MPC-11) (CL647-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60207 targets Galectin-3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Galectin-3 fusion protein Ag6753 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene symbol Galectin-3
Gene ID (NCBI) 3958
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 Galectin-3 antibody CL647-60207Download protocol
Standard Protocols
Click here to view our Standard Protocols