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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Gamma Catenin Monoclonal antibody

Gamma Catenin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, Canine

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5D10

Cat no : CL488-66445

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Gamma Catenin Monocl (CL488-66445)

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated Gamma Catenin Monocl (CL488-66445)

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHT-29 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66445 targets Gamma Catenin in WB applications and shows reactivity with human, rat, pig, Canine samples.

Tested Reactivity human, rat, pig, Canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Gamma Catenin fusion protein Ag1627 相同性解析による交差性が予測される生物種
Full Name junction plakoglobin
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC011865
Gene symbol Gamma Catenin
Gene ID (NCBI) 3728
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 Gamma Catenin antibody CL488-66445Download protocol
Standard Protocols
Click here to view our Standard Protocols