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- KD/KO Validated
CoraLite®555-conjugated Geminin Polyclonal antibody
Geminin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
Cat no : CL555-10802
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL555-10802 targets Geminin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Geminin fusion protein Ag1076 相同性解析による交差性が予測される生物種 |
| Full Name | geminin, DNA replication inhibitor |
| Calculated molecular weight | 24 kDa |
| Observed molecular weight | 28-29 kDa |
| GenBank accession number | BC005185 |
| Gene symbol | GMNN |
| Gene ID (NCBI) | 51053 |
| RRID | AB_2919592 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 Geminin antibody CL555-10802 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep RAD52-dependent mitotic DNA synthesis is required for genome stability in Cyclin E1-overexpressing cells |
