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Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-22270 targets Giantin in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | golgin B1, golgi integral membrane protein |
| Calculated molecular weight | 376 kDa |
| GenBank accession number | NM_004487 |
| Gene Symbol | Giantin |
| Gene ID (NCBI) | 2804 |
| RRID | AB_3084066 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14789 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Giantin is conserved Golgi membrane protein containing a large cytoplasmic domain. It may function to form the intercisternal cross-bridges of the Golgi complex. Anti-Giantin has been widely used to label the Golgi apparatus.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Giantin antibody CL488-22270 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |