Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL555-11037 targets Glutamine Synthetase in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Glutamine Synthetase fusion protein Ag1510 相同性解析による交差性が予測される生物種 |
| Full Name | glutamate-ammonia ligase (glutamine synthetase) |
| Calculated molecular weight | 374 aa, 42 kDa |
| Observed molecular weight | 40-42 kDa |
| GenBank accession number | BC011700 |
| Gene Symbol | Glutamine Synthetase |
| Gene ID (NCBI) | 2752 |
| RRID | AB_3084524 |
| Conjugate | CoraLite® Plus 555 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 554 nm / 570 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15104 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLUL(Glutamine synthetase) also named GS and GLNS, belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667). Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 555 Glutamine Synthetase antibody CL555-11037 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |