• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL488-66323

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Glutamine Synthetase (CL488-66323)

    IF Staining of mouse brain using CL488-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66323 (Glutamine synthetase antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Glutamine Synthetase (CL488-66323)

    IF Staining of mouse brain using CL488-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Glutamine Synthetase antibody (CL488-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated Glutamine Synthetase (CL488-66323)

    FC experiment of HepG2 using CL488-66323

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Glutamine Synthetase (CL488-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66323 targets Glutamine Synthetase in IF-P, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
GenBank accession numberBC011700
Gene symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2883309
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Glutamine Synthetase antibody CL488-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols