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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL594-66323

Synonyms

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Glutamine Synthetase Monoc (CL594-66323)

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Glutamine Synthetase Monoc (CL594-66323)

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®594-conjugated Glutamine Synthetase Monoc (CL594-66323)

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®594-conjugated Glutamine Synthetase Monoc (CL594-66323)

    FC experiment of HepG2 using CL594-66323

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Glutamine Synthetase (CL594-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66323 targets Glutamine Synthetase in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
GenBank accession numberBC011700
Gene symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2883558
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 Glutamine Synthetase antibody CL594-66323Download protocol
FC protocol for CL594 Glutamine Synthetase antibody CL594-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols