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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using GSDMD Recombinant antibody (83562-3-RR)

    WB analysis of NIH/3T3 using 83562-3-RR (same clone as 83562-3-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GSDMD Recombinant antibody (83562-3-RR)

    WB analysis using 83562-3-RR (same clone as 83562-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00534-2

    Sandwich ELISA standard curve of MP00534-2

    Sandwich ELISA standard curve of MP00534-2, Mouse Gsdmd Recombinant Matched Antibody Pair - PBS only. 83562-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34905. 83562-3-PBS was HRP conjugated as the detection antibody. Range: 0.16-10 ng/mL

  • Sample test of MP00534-2

    Sample test of MP00534-2

    J774A.1 call lysate was measured.The mouse Gsdmd concentration of detected samples was determined to be 21.0 ng/mL, based on a 3.4 mg/mL lysate load.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using GSDMD Recombinant antibody (83562-3-RR)

    IHC staining of mouse spleen using 83562-3-RR (same clone as 83562-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83562-3-RR (GSDMD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using GSDMD Recombinant antibody (83562-3-RR)

    IHC staining of mouse spleen using 83562-3-RR (same clone as 83562-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83562-3-RR (GSDMD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using GSDMD Recombinant antibody (83562-3-RR)

    IF Staining of NIH/3T3 using 83562-3-RR (same clone as 83562-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Gsdmd antibody (83562-3-RR, Clone: 240548B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using GSDMD Recombinant antibody (83562-3-RR)

    FC experiment of RAW 264.7 using 83562-3-RR (same clone as 83562-3-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Gsdmd Recombinant antibody (83562-3-RR, Clone:240548B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83562-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00534-1

    Cytometric bead array standard curve of MP00534-1

    Cytometric bead array standard curve of MP00534-1, MOUSE GSDMD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83562-3-PBS. Detection antibody: 83562-1-PBS. Standard: Ag34905. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00534-2

    Cytometric bead array standard curve of MP00534-2

    Cytometric bead array standard curve of MP00534-2, MOUSE GSDMD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83562-2-PBS. Detection antibody: 83562-3-PBS. Standard: Ag34905. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00534-3

    Cytometric bead array standard curve of MP00534-3

    Cytometric bead array standard curve of MP00534-3, MOUSE GSDMD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83562-4-PBS. Detection antibody: 83562-3-PBS. Standard: Ag34905. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83562-3-RR

    Affinity and Kinetic Characterization of 83562-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83562-3-RR against Mouse Gsdmd were performed. The affinity constant is 1.19 nM.

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Mouse GSDMD Recombinant antibody, PBS Only (Capture/Detector)

GSDMD Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83562-3-PBS
Clone No.240548B10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83562-3-PBS targets GSDMD as part of a matched antibody pair:

MP00534-1: 83562-3-PBS capture and 83562-1-PBS detection (validated in Cytometric bead array)

MP00534-2: 83562-2-PBS capture and 83562-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00534-3: 83562-4-PBS capture and 83562-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMD fusion protein Ag34905 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC029813
Gene Symbol Gsdmd
Gene ID (NCBI) 69146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9D8T2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.