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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMD Recombinant antibody

GSDMD Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240548B10

Cat no : 83562-3-RR

Synonyms

Gsdmdc1, gasdermin D, Dfna5l, DF5L, AW558049

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Gsdmd Recombinant antibody (83562-3-RR)

    WB analysis of NIH/3T3 using 83562-3-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Gsdmd Recombinant antibody (83562-3-RR)

    WB analysis using 83562-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83562-3-RR (Gsdmd antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using GSDMD Recombinant antibody (83562-3-RR)

    IF Staining of NIH/3T3 using 83562-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Gsdmd antibody (83562-3-RR, Clone: 240548B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using GSDMD Recombinant antibody (83562-3-RR)

    FC experiment of RAW 264.7 using 83562-3-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Gsdmd Recombinant antibody (83562-3-RR, Clone:240548B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83562-3-RR

    Affinity and Kinetic Characterization of 83562-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83562-3-RR against Mouse Gsdmd were performed. The affinity constant is 1.19 nM.

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Tested Applications

Positive WB detected inNIH/3T3 cells, EL-4 cells, RAW 264.7 cells, J774A.1 cells
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83562-3-RR is GSDMD Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GSDMD fusion protein Ag34905 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC029813
Gene symbol Gsdmd
Gene ID (NCBI) 69146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 83562-3-RRDownload protocol
IF protocol for GSDMD antibody 83562-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols