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HA tag Polyclonal antibody

HA tag Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 51064-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

HA, HA tag, YPYDVPDYA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51064-2-AP targets HA tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name HA tag
Calculated molecular weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_11042321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Protocols

Product Specific Protocols
WB protocol for HA tag antibody 51064-2-APDownload protocol
IF protocol for HA tag antibody 51064-2-APDownload protocol
IP protocol for HA tag antibody 51064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
WB

Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei
WB,CoIP

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
WB

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
WB

Cell

RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.

Authors - Lara Rheinemann
WB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
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