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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite® Plus 488-conjugated HA tag Polyclonal an (CL488-51064)

    IF Staining of Transfected HEK-293 using CL488-51064

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 HA tag antibody (CL488-51064) at dilution of 1:200.

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CoraLite® Plus 488-conjugated HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF/ICC
Cat No. CL488-51064

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-51064 targets HA tag in IF/ICC applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name HA tag
Calculated molecular weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2935603
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HA tag antibody CL488-51064Download protocol
Standard Protocols
Click here to view our Standard Protocols