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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated HABP2 Monoclonal ant (CL488-67867)

    IF Staining of HepG2 using CL488-67867

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HABP2 antibody (CL488-67867, Clone: 1A7C2 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated HABP2 Monoclonal ant (CL488-67867)

    FC experiment of HepG2 using CL488-67867

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated HABP2 Monoclonal antibody (CL488-67867, Clone:1A7C2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated HABP2 Monoclonal antibody

HABP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67867
Clone No.1A7C2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

FSAP, Factor VII-activating protease, Factor VII activating protease, Factor seven-activating protease, EC:3.4.21.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67867 targets HABP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HABP2 fusion protein Ag3981 相同性解析による交差性が予測される生物種
Full Name hyaluronan binding protein 2
Calculated molecular weight 560 aa, 63 kDa
Observed molecular weight60,63 kDa
GenBank accession numberBC031412
Gene Symbol HABP2
Gene ID (NCBI) 3026
RRIDAB_3672965
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14520
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HABP2 is also named as HGFAL, PHBP and belongs to the peptidase S1 family. It can activate coagulation factor VII and prourokinase and release the vasoactive peptide bradykinin by cleaving kininogen and is an important role in the regulation of the haemostasis system as well as fibroproliferative inflammatory processes (PMID:21655671). HABP2 is present as the 70 kDa inactive single chain precursor in human plasma and transforms to the active two chain form, 50 kDa heavy chain and 27 kDa light chain,and the 50 kDa heavy chain changes to two 26 kDa fragments, and 27 kDa light chain to 17 kDa and 8 kDa fragments, which are all bridged by disulfide linkages, by autocleavage(PMID:11217080).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HABP2 antibody CL488-67867Download protocol
FC protocol for CL Plus 488 HABP2 antibody CL488-67867Download protocol
Standard Protocols
Click here to view our Standard Protocols