HADHB Monoclonal antibody
HADHB Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Pig
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D12F4
Cat no : 67967-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, pig heart tissue, Hela cells, HEK-293 cells, HepG2 cells, Jurkat cells, Rabbit heart tissues, Rat heart tissues, Mouse heart tissues |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67967-1-Ig targets HADHB in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Reactivity | Human, Mouse, Rat, Pig |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | HADHB fusion protein Ag30315 相同性解析による交差性が予測される生物種 |
Full Name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC017564 |
Gene symbol | HADHB |
Gene ID (NCBI) | 3032 |
RRID | AB_2918718 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for HADHB antibody 67967-1-Ig | Download protocol |
IHC protocol for HADHB antibody 67967-1-Ig | Download protocol |
IF protocol for HADHB antibody 67967-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |