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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HADHB Monoclonal antibody, PBS Only

HADHB Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12F4

Cat no : 67967-1-PBS

Synonyms

Acetyl CoA acyltransferase, Beta ketothiolase, ECHB, HADHB, MSTP029, TP BETA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HADHB Monoclonal antibody (67967-1-Ig)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HADHB Monoclonal antibody (67967-1-Ig)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HADHB Monoclonal antibody (67967-1-Ig)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HADHB Monoclonal antibody (67967-1-Ig)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HADHB Monoclonal antibody (67967-1-Ig)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HADHB Monoclonal antibody (67967-1-Ig)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67967-1-PBS targets HADHB in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HADHB fusion protein Ag30315 相同性解析による交差性が予測される生物種
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
Calculated molecular weight 51 kDa
Observed molecular weight47 kDa
GenBank accession numberBC017564
Gene symbol HADHB
Gene ID (NCBI) 3032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.