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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HADHB Monoclonal antibody, PBS Only (Capture)

HADHB Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12F4

Cat no : 67967-1-PBS

Synonyms

Beta-ketothiolase, Beta ketothiolase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, 3-ketoacyl-CoA thiolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HADHB Monoclonal antibody (67967-1-Ig)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HADHB Monoclonal antibody (67967-1-Ig)

    WB analysis using 67967-1-Ig (same clone as 67967-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67967-1-Ig (HADHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HADHB Monoclonal antibody (67967-1-Ig)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HADHB Monoclonal antibody (67967-1-Ig)

    IHC staining of human colon cancer using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67967-1-Ig (HADHB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HADHB Monoclonal antibody (67967-1-Ig)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HADHB Monoclonal antibody (67967-1-Ig)

    IF Staining of HeLa using 67967-1-Ig (same clone as 67967-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HADHB antibody (67967-1-Ig, Clone: 1D12F4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67967-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50546-1

    Cytometric bead array standard curve of MP50546-1

    Cytometric bead array standard curve of MP50546-1, HADHB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67967-1-PBS. Detection antibody: 67967-2-PBS. Standard:Ag30315. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67967-1-PBS targets HADHB as part of a matched antibody pair:

MP50546-1: 67967-1-PBS capture and 67967-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HADHB fusion protein Ag30315 相同性解析による交差性が予測される生物種
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
Calculated molecular weight 51 kDa
Observed molecular weight47 kDa
GenBank accession numberBC017564
Gene symbol HADHB
Gene ID (NCBI) 3032
RRIDAB_2918718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HADHB, also named as TP- beta, Acetyl-CoA acyltransferase and Beta-ketothiolase, is a mitochondrial trifunctional enzyme subunit beta. Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity. HADHB has 2 isoforms produced by alternative splicing with the MW of 49 kDa and 51 kDa.