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HAX1 Monoclonal antibody

HAX1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2A12F11

Cat no : 67883-2-Ig

Synonyms

FLJ17042, FLJ18492, FLJ93803, HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAX1 Monoclonal antibody (67883-2-Ig)

    WB analysis using 67883-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HAX1 Monoclonal antibody (67883-2-Ig)

    WB analysis of HEK-293 using 67883-2-Ig

    WB result of HAX1 antibody (67883-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HAX1 Monoclonal antibody (67883-2-Ig)

    IF Staining of HeLa using 67883-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

CoIPSee 1 publications below

Product Information

67883-2-Ig targets HAX1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HAX1 fusion protein Ag27244 相同性解析による交差性が予測される生物種
Full Name HCLS1 associated protein X-1
Calculated molecular weight 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC014314
Gene symbol HAX1
Gene ID (NCBI) 10456
RRIDAB_2918640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAX1 antibody 67883-2-IgDownload protocol
IF protocol for HAX1 antibody 67883-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Phytomedicine

Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts

Authors - Tingting Zhang
View Article