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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAX1 Monoclonal antibody (67883-2-Ig)

    WB analysis using 67883-2-Ig (same clone as 67883-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67883-2-Ig (HAX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HAX1 Monoclonal antibody (67883-2-Ig)

    WB analysis of HEK-293 using 67883-2-Ig (same clone as 67883-2-PBS)

    WB result of HAX1 antibody (67883-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HAX1 Monoclonal antibody (67883-2-Ig)

    IF Staining of HeLa using 67883-2-Ig (same clone as 67883-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HAX1 antibody (67883-2-Ig, Clone: 2A12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67883-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50775-2

    Cytometric bead array standard curve of MP50775-2

    Cytometric bead array standard curve of MP50775-2, HAX1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67883-2-PBS. Detection antibody: 67883-5-PBS. Standard:Ag27244. Range: 0.391-50 ng/mL.

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  • KD/KO Validated

HAX1 Monoclonal antibody, PBS Only (Capture)

HAX1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67883-2-PBS
Clone No.2A12F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

HS1-associating protein X-1, HCLSBP1, HCLS1-associated protein X-1, HAX-1, HAX 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67883-2-PBS targets HAX1 as part of a matched antibody pair:

MP50775-2: 67883-2-PBS capture and 67883-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HAX1 fusion protein Ag27244 相同性解析による交差性が予測される生物種
Full Name HCLS1 associated protein X-1
Calculated molecular weight 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC014314
Gene Symbol HAX1
Gene ID (NCBI) 10456
RRIDAB_2918640
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00165
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.