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- KD/KO Validated
Hemoglobin Alpha Polyclonal antibody
Hemoglobin Alpha Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 14537-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, mouse heart tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IF-P detected in | human appendicitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14537-1-AP targets Hemoglobin Alpha in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hemoglobin Alpha fusion protein Ag6040 相同性解析による交差性が予測される生物種 |
| Full Name | hemoglobin, alpha 1 |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 14-16 kDa, 27 kDa |
| GenBank accession number | BC050661 |
| Gene symbol | HBA1 |
| Gene ID (NCBI) | 3039 |
| RRID | AB_2114462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| IF protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| IP protocol for Hemoglobin Alpha antibody 14537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Int J Mol Sci Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus.
| ||
Haematologica Therapeutic effects of induced pluripotent stem cells in chimeric mice with beta-thalassemia. | ||
Nutrients Cellular Zinc Deficiency Impairs Heme Biosynthesis in Developing Erythroid Progenitors | ||
Arch Biochem Biophys SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes. |
