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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Hemoglobin Alpha Polyclonal antibody

Hemoglobin Alpha Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14537

Synonyms

HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CoraLite®594-conjugated Hemoglobin Alpha Polyclona (CL594-14537)

    IF Staining of human appendicitis using CL594-14537

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 Hemoglobin Alpha antibody (CL594-14537) at dilution of 1:200.

  • Flow cytometry (FC) experiment of TF-1 cells using CoraLite®594-conjugated Hemoglobin Alpha Polyclona (CL594-14537)

    FC experiment of TF-1 using CL594-14537

    1X10^6 TF-1 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Hemoglobin Alpha (CL594-14537) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inTF-1 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14537 targets Hemoglobin Alpha in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hemoglobin Alpha fusion protein Ag6040 相同性解析による交差性が予測される生物種
Full Name hemoglobin, alpha 1
Calculated molecular weight 15 kDa
Observed molecular weight 14-16 kDa, 27 kDa
GenBank accession numberBC050661
Gene symbol HBA1
Gene ID (NCBI) 3039
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

Protocols

Product Specific Protocols
IF protocol for CL594 Hemoglobin Alpha antibody CL594-14537Download protocol
Standard Protocols
Click here to view our Standard Protocols