• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human placenta tissue using Hemoglobin Alpha Recombinant antibody (83185-5-RR)

    WB analysis of human placenta using 83185-5-RR (same clone as 83185-5-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using Hemoglobin Alpha Recombinant antibody (83185-5-RR)

    FC experiment of K-562 using 83185-5-RR (same clone as 83185-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of TF-1 cells using Hemoglobin Alpha Recombinant antibody (83185-5-RR)

    FC experiment of TF-1 using 83185-5-RR (same clone as 83185-5-PBS)

    1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83185-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83185-5-RR

    Affinity and Kinetic Characterization of 83185-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

View 3D epitope

Hemoglobin Alpha Recombinant antibody, PBS Only

Hemoglobin Alpha Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83185-5-PBS
Clone No.240049F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【PR】一次抗体まとめて特別価格！『PRIME BUY抗体特割キャンペーン』4/21スタート

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83185-5-PBS targets Hemoglobin Alpha in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Hemoglobin Alpha fusion protein Ag6040 相同性解析による交差性が予測される生物種
Full Name hemoglobin, alpha 1
Calculated molecular weight 15 kDa
Observed molecular weight14-16 kDa, 27 kDa
GenBank accession numberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP69905
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.