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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HBA1 Recombinant antibody (83185-5-RR)

    WB analysis of human placenta using 83185-5-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of K-562 cells using Hemoglobin Alpha Recombinant antibody (83185-5-RR)

    FC experiment of K-562 using 83185-5-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of TF-1 cells using Hemoglobin Alpha Recombinant antibody (83185-5-RR)

    FC experiment of TF-1 using 83185-5-RR

    1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83185-5-RR

    Affinity and Kinetic Characterization of 83185-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

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Hemoglobin Alpha Recombinant antibody

Hemoglobin Alpha Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83185-5-RR
Clone No.240049F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive FC (Intra) detected inK-562 cells, TF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83185-5-RR targets Hemoglobin Alpha in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Hemoglobin Alpha fusion protein Ag6040 相同性解析による交差性が予測される生物種
Full Name hemoglobin, alpha 1
Calculated molecular weight 15 kDa
Observed molecular weight14-16 kDa, 27 kDa
GenBank accession numberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
RRIDAB_3670874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP69905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

Protocols

Product Specific Protocols
WB protocol for Hemoglobin Alpha antibody 83185-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols