Hemoglobin Epsilon Polyclonal antibody
Hemoglobin Epsilon Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 12361-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, K-562 cells, mouse placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
12361-1-AP targets Hemoglobin Epsilon in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hemoglobin Epsilon fusion protein Ag3026 相同性解析による交差性が予測される生物種 |
| Full Name | hemoglobin, epsilon 1 |
| Calculated molecular weight | 147 aa, 16 kDa |
| Observed molecular weight | 12-16 kDa |
| GenBank accession number | BC015537 |
| Gene symbol | HBE1 |
| Gene ID (NCBI) | 3046 |
| RRID | AB_2114593 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Hemoglobin Epsilon antibody 12361-1-AP | Download protocol |
| IHC protocol for Hemoglobin Epsilon antibody 12361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Blood NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression. | ||
Cell Death Differ Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors. | ||
Mol Cell Biol A Feedback Loop Consisting of MicroRNA 23a/27a and the β-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression. | ||
Biochim Biophys Acta Mol Cell Res Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins. | ||
