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Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®555-conjugated Hemoglobin Epsilon Polyclo (CL555-12361)

    FC experiment of K-562 using CL555-12361

    1x10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated Hemoglobin Epsilon Polyclonal antibody (CL555-12361)(red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite®555-conjugated Hemoglobin Epsilon Polyclonal antibody

Hemoglobin Epsilon Polyclonal Antibody for FC (Intra)
Cat No. CL555-12361

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

HBE1, Hemoglobin epsilon-Specific, Hemoglobin epsilon chain, HBE, Epsilon-globin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-12361 targets Hemoglobin Epsilon in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hemoglobin Epsilon fusion protein Ag3026 相同性解析による交差性が予測される生物種
Full Name hemoglobin, epsilon 1
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 12-16 kDa
GenBank accession numberBC015537
Gene Symbol HBE1
Gene ID (NCBI) 3046
RRIDAB_2919609
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02100
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).

Protocols

Product Specific Protocols
FC protocol for CL555 Hemoglobin Epsilon antibody CL555-12361Download protocol
Standard Protocols
Click here to view our Standard Protocols