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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HDAC10 Monoclonal antibody

HDAC10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1B11

Cat no : 67646-1-Ig

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC10 Monoclonal antibody (67646-1-Ig)

    WB analysis using 67646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using HDAC10 Monoclonal antibody (67646-1-Ig)

    WB analysis using 67646-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using HDAC10 Monoclonal antibody (67646-1-Ig)

    IF Staining of Jurkat using 67646-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HDAC10 Monoclonal antibody (67646-1-Ig)

    IF Staining of MCF-7 using 67646-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67646-1-Ig targets HDAC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HDAC10 fusion protein Ag18470 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 10
Calculated molecular weight 669 aa, 71 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC125083
Gene symbol HDAC10
Gene ID (NCBI) 83933
RRIDAB_2882846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

Protocols

Product Specific Protocols
WB protocol for HDAC10 antibody 67646-1-IgDownload protocol
IF protocol for HDAC10 antibody 67646-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Cancer Res

Molecular, biological characterization and drug sensitivity of chidamide-resistant MCF7 cells

Authors - Liya Dai
View Article