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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC10 Monoclonal antibody (67646-1-Ig)

    WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HDAC10 Monoclonal antibody (67646-1-Ig)

    WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HDAC10 Monoclonal antibody (67646-1-Ig)

    IF Staining of MCF-7 using 67646-1-Ig (same clone as 67646-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using HDAC10 Monoclonal antibody (67646-1-Ig)

    IF Staining of Jurkat using 67646-1-Ig (same clone as 67646-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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HDAC10 Monoclonal antibody, PBS Only

HDAC10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67646-1-PBS
Clone No.1A1B11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Polyamine deacetylase HDAC10, HDAC 10, HD10, HD 10, EC:3.5.1.48

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67646-1-PBS targets HDAC10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HDAC10 fusion protein Ag18470 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 10
Calculated molecular weight 669 aa, 71 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC125083
Gene Symbol HDAC10
Gene ID (NCBI) 83933
RRIDAB_2882846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969S8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.