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CoraLite® Plus 647-conjugated HDAC2 Monoclonal antibody

HDAC2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A3E4

Cat no : CL647-67165

Synonyms

HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1


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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67165 targets HDAC2 in IF/ICC applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag21288 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 2
Calculated molecular weight458 aa, 52 kDa; 488 aa,55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
RRIDAB_2920291
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 HDAC2 antibody CL647-67165Download protocol
FC protocol for CL Plus 647 HDAC2 antibody CL647-67165Download protocol
Standard Protocols
Click here to view our Standard Protocols