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  • Featured Product
  • KD/KO Validated

HDAC8 Polyclonal antibody, PBS Only

HDAC8 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17548-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using HDAC8 Polyclonal antibody (17548-1-AP)

    WB analysis of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using HDAC8 Polyclonal antibody (17548-1-AP)

    WB analysis of Raji using 17548-1-AP (same clone as 17548-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using HDAC8 Polyclonal antibody (17548-1-AP)

    IP experiment of Raji using 17548-1-AP (same clone as 17548-1-PBS)

    IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC8 Polyclonal antibody (17548-1-AP)

    IF Staining of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17548-1-PBS targets HDAC8 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC8 fusion protein Ag11696 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 8
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC050433
Gene symbol HDAC8
Gene ID (NCBI) 55869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.