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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis of HEK-293 using 83643-4-RR (same clone as 83643-4-PBS)

    WB result of HEATR3 antibody (83643-4-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEATR3 transfected HEK-293 cells. This data was developed using the same antibody clone with 83643-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis using 83643-4-RR (same clone as 83643-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83643-4-RR (HEATR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83643-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83643-4-RR

    Affinity and Kinetic Characterization of 83643-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83643-4-RR against Human HEATR3 were performed. The affinity constant is 0.657 nM.

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  • KD/KO Validated

HEATR3 Recombinant antibody, PBS Only

HEATR3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83643-4-PBS
Clone No.240561F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3, 240561F7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83643-4-PBS targets HEATR3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEATR3 fusion protein Ag26328 相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 3
Calculated molecular weight 680 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC033077
Gene Symbol HEATR3
Gene ID (NCBI) 55027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z4Q2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.