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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis of HEK-293 using 83643-4-RR

    WB result of HEATR3 antibody (83643-4-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEATR3 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis using 83643-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83643-4-RR (HEATR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83643-4-RR

    Affinity and Kinetic Characterization of 83643-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83643-4-RR against Human HEATR3 were performed. The affinity constant is 0.657 nM.

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HEATR3 Recombinant antibody

HEATR3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83643-4-RR
Clone No.240561F7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3, 240561F7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 ug/ml
20 μL, 1000 ug/ml
100 μL, 1000 ug/ml

¥85,000/ 100 μL

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83643-4-RR targets HEATR3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEATR3 fusion protein Ag26328 相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 3
Calculated molecular weight 680 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC033077
Gene Symbol HEATR3
Gene ID (NCBI) 55027
RRIDAB_3671253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z4Q2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HEATR3 antibody 83643-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols