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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HEATR3 Recombinant antibody

HEATR3 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240561F7

Cat no : 83643-4-RR

Synonyms

HEAT repeat-containing protein 3, HEAT repeat containing 3, 240561F7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HEATR3 Recombinant antibody (83643-4-RR)

    WB analysis using 83643-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83643-4-RR (HEATR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83643-4-RR

    Affinity and Kinetic Characterization of 83643-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83643-4-RR against Human HEATR3 were performed. The affinity constant is 0.657 nM.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83643-4-RR is HEATR3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HEATR3 fusion protein Ag26328 相同性解析による交差性が予測される生物種
Full Name HEAT repeat containing 3
Calculated molecular weight 680 aa, 75 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC033077
Gene symbol HEATR3
Gene ID (NCBI) 55027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HEATR3 antibody 83643-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols