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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HEC1/HEC/NDC80 Recombinant antibody

HEC1/HEC/NDC80 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5A2

Cat no : CL488-83859

Synonyms

NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated HEC1/HEC/NDC80 Recom (CL488-83859)

    IF Staining of HeLa using CL488-83859

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HEC1/HEC/NDC80 antibody (CL488-83859, Clone: 5A2 ) at dilution of 1:200, CoraLite®594 Alpha Tubulin antibody (CL594-66031, Clone: 1E4C11, red).

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83859 is HEC1/HEC/NDC80 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Observed molecular weight 74 kDa
GenBank accession number/
Gene symbol NDC80
Gene ID (NCBI) 10403
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HEC1/HEC/NDC80 antibody CL488-83859Download protocol
Standard Protocols
Click here to view our Standard Protocols